Measuring Disease and Development in Live Cell and Tissue with 5-D Microscopy

Friday, January 15, 2016

4:00 PM-5:30 PM

BIOMED Seminar

Title:
Measuring Disease and Development in Live Cell and Tissue with 5-D Microscopy Speaker: Andrew R. Cohen, PhD Associate Professor Department of Electrical and Computer Engineering Drexel University
 
Details:
Time-lapse microscopy of live cells and tissue captures “5-D” movies of living cells and subcellular organelles. These 5-D image sequences consist of 2 or 3 spatial dimensions plus time and multiple imaging channels captured under different conditions of disease and development. Optical imaging allows the long term observation of complex biological events including the development of clones (family trees) of stem or cancer cells. This data captures cellular patterns of motion, morphology, and appearance and clonal, or lineage, information such as cell cycle times and progeny fate.

There is also the opportunity to visualize the dynamics of sub-cellular organelles, all in the intact tissue microenvironment. This rich and complex view of the dynamic processes of disease and development requires computational tools that enable rigorous quantification and also allow human exploration and interaction with the data. This talk will describe computational image analysis approaches for “summarizing” 5-D image sequence data using denoising, segmentation, tracking and lineaging algorithms. Visualization plays a key role in the analysis, allowing the user to explore the full range of image data together interactively with the summarization.

New tools built on the HTML 5 application framework make ‘all' of the image and summarization results available on any modern computing device – game changing advances for publishing and reproducibility. Image data and results from 2-D and 3-D time-lapse microscopy with multiple fluorescence channels will be shown from applications including human cancer cells, mouse embryonic and adult neural stem cell development, proliferating T lymphocytes and macrophage/TB interaction in HIV+ populations.

For more info, please visit drexel.edu/biomed. 

Biosketch:
Dr. Andrew Cohen joined the faculty in the department of electrical and computer engineering at Drexel University as an associate professor in August 2012. Before coming to Drexel, he was an assistant professor at the University of Wisconsin-Milwaukee. He received his PhD from the Rensselaer Polytechnic Institute in May 2008 working with Professor Badri Roysam. His postdoctoral research on developing computational approaches to quantifying deficiencies in axonal organelle transport due to neurodegenerative disease was funded by the Huntington's Disease Foundation.

Contact Information

Ken Barbee
215-895-1335
barbee@drexel.edu