For a better experience, click the Compatibility Mode icon above to turn off Compatibility Mode, which is only for viewing older websites.

Research in the Romano lab is focused on studying cancer drug resistance using a conjunction of mouse modeling, functional genomics and computational biology. We study intrinsic and acquired molecular mechanisms by which tumor cells resist targeted and/or immunotherapy approaches, with the final aim of developing novel therapeutic strategies for cancer patients. We are particularly interested in:

The tumor-stroma immunological axis in minimal residual disease

Multiplex staining of immune cells and chemokines in mouse tumor samples. Source: Romano Lab

Multiplex staining of immune cells and chemokines in mouse tumor samples.

When a small group of drug-tolerant cells evades treatment, it can give rise to a minimal residual disease, where tumor cells linger in a dormant state. As minimal residual disease can fuel relapse and metastasis, its eradication is fundamental to obtain long-lasting clinical effects. We are currently optimizing chemokine-based approaches to influence the composition of the immune infiltrate and direct the immune system towards eliminating residual tumor cells.

The role of tumor suppressors in cancer drug resistance

Patient relapse. The role of tumor suppressors in cancer drug resistance. Source: Romano Lab

PET-scan of a metastatic melanoma patient who initially responded to therapy but eventually developed resistance.

Most of the current targeted therapy approaches inhibit oncogene activity (i.e. BRAF inhibitors, EGFR inhibitors), often obtaining impressive clinical responses. Nevertheless, cancer is a genetic illness where at least 2-3 tumor suppressor gene mutations are usually required for tumorigenesis. Our lab uses functional genomics experiments to detect and anticipate tumor-suppressor-mediated resistance mechanisms to targeted therapy. We aim to develop effective counter-therapies to administer in conjunction or substitution to current standard of care approaches.

The Romano lab has experience in:

  • Mouse models of cancer (GEMM, xenografts, syngeneic)
  • Biological validation of targets through functional genomics (knock-in, knock-out, CRISPR, Cre-LoxP)
  • Drug and genetic screening to identify actionable targets of drug sensitivity (CRISPR screens, chemical library screens)
  • Micro- and nano-particle delivery of immunomodulating compounds
  • Bioinformatic analysis of "omic" data (RNAseq, WES, RPPA) on patient and mouse samples
  • Collaborations across the US and global institutions for coordinated development of translational projects

Bioinformatic deconvolution of tumor immune infiltrate before and after treatment. Source: Romano Lab
Bioinformatic deconvolution of tumor immune infiltrate before and after treatment.

Open Position

The Romano lab is currently looking for a motivated research assistant willing to contribute to the scientific growth of the laboratory. Click here for additional information. Students (grad and undergrad) and potential collaborators are always welcome to contact Dr. Romano for questions, ideas and suggestions.

View Romano Lab Publications

 
 Back to Top