The Drexel University College of Medicine Department of Microbiology & Immunology includes:
Laboratory for Protein Science/Flow
Director: Allison Carey, MD
Drexel University College of Medicine's Laboratory for Protein Science is housed in the Department of Microbiology & Immunology and consists of both flow cytometry and deconvolution microscopy. The flow facility is located in Room G41 at the Queen Lane Campus. This facility contains two instruments that can be used for clinical and basic research and are available to the research community as a shared resource. The flow facility is equipped with a bench-top FACSCalibur Flow Cytometer (BD Biosciences, San Jose, California) and a FACSAria (BD Biosciences) high-speed cell sorter. See descriptor below of the two instruments for their capabilities. Please contact the staff of the flow facility for advice, protocols, sample preparation and pricing (215.991.8372). Please make reservations at least 48 hours prior to expected use.
Calibur: This modular flow cytometer is equipped with a dual laser sensor unit, a sample loader, and a FACStation data management system with a Macintosh computer and printer. The cytometer is equipped with a 488nm and 633nm lasers and can detect four colors: FITC, Phycoerythrin (PE), PerCP or PE-Cy5 (Cychrome), and allophycocyanin (APC). The cytometer can identify various cell types using multiple parameters including forward light scatter, side light scatter, and multicolor analysis (up to four colors simultaneously), which can be analyzed using Cell Quest and FlowJo software. The cytometer can also measure DNA content using Modfit LT software.
FACSAria: The FACSAria cell sorter (BD Biosciences, San Jose, CA) is a state-of-the-art high-performance flow cell based FACS sorter equipped with three lasers (488nm, 633nm and 407nm lasers) and capable of high-speed cell sorting. The sorter is equipped with a FacsAria Workstation for data acquisition, aerosol management and temperature control. Multicolor analysis (15 parameter) and high-speed sorting (up to 70,000 cell/sec) of target cell populations can be performed. The sorter can analyze up to 15 parameters simultaneously including forward light scatter, side light scatter, and multicolor analysis. The sorter is currently equipped with detectors for FITC, PE, PerCP or PE-Cy5, PE-Cy5.5, PE-Cy7, APC, APC-Cy7, Alexa 594, APC-Cy5.5, Alexa 430, Cascade blue.
Data can be analyzed using Cell Quest and FlowJo software. The cytometer can also measure DNA content using Modfit LT software.
Deconvolution Microscopy: The microscopy facility includes an Olympus IX81 inverted fluorescent deconvolution microscopy system with Slidebook deconvolution imaging software.
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