Drexel Researchers Identify 'Master Regulator' Involved in Infant Lung Damage
October 27, 2017
Researchers at Drexel University have revealed that inhibiting the micro-RNA miR-34a significantly reduces bronchopulmonary dysplasia (BPD) in mice. BPD is a chronic lung disease that affects up to 15,000 premature infants every year in the United States. The study, published in Nature Communications, suggests that targeting this “master regulator” (miR-34a) could treat this complex disorder.
BPD develops when premature infants with underdeveloped lungs need help to breathe and are treated with supplemental oxygen. Though the oxygen is life-saving, it often leads to inflammation and injury in the lungs, which can cause life-long breathing problems.
Vineet Bhandari, MD, a faculty member in the Department of Pediatrics at Drexel University’s College of Medicine and chief of Neonatal Medicine at St. Christopher’s Hospital for Children, suspected that the microRNA miR-34a could play a role in BPD. Previous studies have suggested that the microRNA molecule controls pathways related to four major aspects of the disease: inflammation, vascular development, cell death and cell proliferation.
The lung tissues of mice exposed to high concentrations of oxygen (hyperoxia), which mimics the most common chronic human lung disease in infants, bronchopulmonary dysplasia (BPD). The large and simplified air sacs in the control BPD lungs (left) significantly improved toward a normal size and shape in the lungs administered with the miR-34a-inhibitor (right).
To investigate the theory, Bhandari and colleagues first tested a cohort of lung secretion samples taken from premature infants. After testing the samples, the researchers found that miR-34a was significantly increased only in the samples taken from babies who went on to develop BPD.
The researchers also tested lung tissue collected postmortem from babies who had died at different ages from lung disease. Again, they found that this particular microRNA was expressed in the samples and also its downstream targets (the production of proteins) were suppressed in the ones with BPD.
Next, the researchers found that levels of miR-34a levels were increased in the lungs of neonatal mice exposed to high levels of oxygen. Bhandari and his team then blocked the microRNA by repressing gene expression, as well as pharmacologically, by injecting the mouse models with the downstream target of the miR-34a, angiopoietin-1. Blocking miR-34a or injecting angiopoietin-1 in the mouse models improved their lung architecture by up to 90 percent, compared to controls.
“People have been looking to find a master regulator involved in BPD, and many thought it could not be found, because it is such a complicated disease,” Bhandari said. “But we have comprehensively proven that miR-34a controls at least four different pathways involved in BPD.”
The researchers are pinpointing new targets for treating BPD and moving toward clinical trials to test new treatments for the disease.
Investigators from Yale University, Thomas Jefferson University, University of Helsinki, University of Alabama at Birmingham and University of Rochester contributed to this work.