Bio
Alisa Morss Clyne, PhD, is a Professor in the Fischell Department of Bioengineering at the University of Maryland's A. James Clark School of Engineering. Dr. Clyne is director of the Vascular Kinetics Laboratory, which investigates integrated mechanical and biochemical interactions among cells and proteins of the cardiovascular system. She is particularly interested in how endothelial cell mechanotransduction changes in a diseased environment, and how fluid shear stress and substrate mechanics affect biochemical binding kinetics, transport, and signaling.
Dr. Clyne was inducted into the American Institute for Medical and Biological Engineering (AIMBE) College of Fellows in 2019. She received the NSF CAREER award in 2008, an AHA National Scientist Development Grant in 2010, and the BMES-CMBE Rising Star award in 2011. She has received research and educational funding from NSF, NIH, AHA, Department of Education, the Nanotechnology Institute, and the State of Pennsylvania, and she has published in diverse journals including Lab on a Chip, Journal of Biomechanics, Annals of Biomedical Engineering, Tissue Engineering, Biophysical Journal, PLOS One, The Journal of Biological Chemistry, and Circulation. She is a fellow of ASME and the AHA, and a member of ASEE, BMES, NAVBO, and SWE.
She received a BS in Mechanical Engineering from Stanford University in 1996, an MS in Mechanical Engineering from the University of Cincinnati in 1999 and her PhD in Medical and Mechanical Engineering from Harvard-MIT Division of Health Sciences and Technology in 2006.
Education
- PhD, Medical and Mechanical Engineering, Massachusetts Institute of Technology (MIT), 2006
- MS, Mechanical Engineering, University of Cincinnati, 1999
- BS, Mechanical Engineering, Stanford University, 1996
Research Interests
Dr. Clyne's Vascular Kinetics Laboratory conducts research at the interface of engineering, biochemistry, and vascular biology. In their prior work, the group largely focused on how an altered glucose environment (e.g., in diabetes) impaired endothelial cell response to shear stress and cyclic strain. Dr. Clyne and her lab have demonstrated that endothelial cells in high glucose do not respond appropriately to mechanical stimuli. Today, the group focuses primarily on the opposite question: how the mechanical environment affects endothelial cell glucose metabolism. They are integrating mechanobiology and metabolism, with the hope of using metabolic engineering to decrease the burden of cardiovascular disease and cancer. With new collaboration opportunities at the University of Maryland, Dr. Clyne and her lab group plan to extend their cell and animal work into human studies.
Publications
JOURNAL PUBLICATIONS
Mathew J, Morss Clyne A. Fibroblast growth factor-2 does not rescue plasminogen system activity or capillary-like tube formation in endothelial cells on glycated collagen. (In review)
Figueroa D, Kemeny S, Morss Clyne A. (In press) Glycated collagen decreased extracellular matrix fibronectin alignment in response to cyclic stretch via interruption of actin alignment. Journal of Biomechanical Engineering
Kemeny S, Figueroa D, Morss Clyne A. (2013) Hypo- and hyperglycemia impair endothelial cell actin alignment and nitric oxide synthase activation in response to shear stress. PLoSOne, 8(6): e66176.
Kemeny S, Cicalese S, Figueroa D, Morss Clyne A. (2013) Glycated collagen and altered glucose increase endothelial cell adhesion strength. Journal of Cellular Physiology, 228 (8): p. 1727-36.
Arjunan K, Morss Clyne A. (2013) A nitric oxide producing pin-to-hole spark discharge plasma enhances endothelial cell proliferation and migration. Plasma Medicine 1(3-4): 279-293. Patel N, Reisig K, Morss Clyne A. (2013) A computational model of fibroblast growth factor-2 binding in fluid flow. Annals of Biomedical Engineering 41(1): p. 154-171.
Morss Clyne A, FitzGerald GA. (2012) What great creation. Science Translational Medicine 4(154).
Yu M, Huang S, Yu KJ, Morss Clyne A. (2012) Dextran and polymer polyethylene glycol (PEG) coating reduce both 5 and 30 nm iron oxide nanoparticle cytotoxicity in 2D and 3D cell culture. International Journal of Molecular Sciences 13(5): p. 5554-70.
Arjunan K, Friedman G, Fridman A, Morss Clyne A. (2012) Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species. Journal of the Royal Society Interface 9(66): p. 147-157.
Arjunan K, Morss Clyne A. (2011) Hydroxyl radical and hydrogen peroxide are primarily responsible for dielectric barrier discharge plasma-induced angiogenesis. Plasma Processes and Polymers 8: p. 1154-64
Kemeny S, Figueroa D, Andrews A, Barbee K, Morss Clyne A. (2011) Glycated collagen alters endothelial cell actin alignment and nitric oxide release in response to fluid shear stress. Journal of Biomechanics 44(10): p. 1927-35.
Figueroa D, Kemeny S, Morss Clyne A. (2011) Glycated collagen impairs endothelial cell response to cyclic stretch. Cellular and Molecular Bioengineering 4 (2): p. 220-30. Kemeny S, Morss Clyne A. (2011) A simplified implementation of edge detection in MATLAB is faster and more sensitive than Fast Fourier Transform for actin fiber alignment quantification. Microscopy and Microanalysis 17: p. 156-166.
Dobrynin D, Arjunan KP, Fridman A, Friedman G, Morss Clyne A. (2011) Direct and controllable nitric oxide delivery into biological media and living cells by a pin-to-hole spark discharge (PHD) plasma. Journal of Physics D: Applied Physics 44: 075201. Buyukhatipoglu K, Morss Clyne A.(2011) Superparamagnetic iron oxide nanoparticles change endothelial cell morphology and mechanics via reactive oxygen species formation. Journal of Biomedical Materials Research Part A 96A (1): p. 186-195.
Morss Clyne A. Thermal processing of tissue engineering scaffolds. (2011) ASME Journal of Heat Transfer Special Issue on Advanced Thermal Processing 133(3): 034001.
Reisig K, Morss Clyne A. (2010) Fibroblast growth factor-2 binding to the endothelial basement membrane peaks at a physiologically relevant shear stress. Matrix Biology 29 (7): p. 586-93.
Buyukhatipoglu K, Chang R, Sun W, Morss Clyne A. (2010) Bioprinted Nanoparticles for Tissue Engineering Applications. Tissue Engineering 16 (4): p. 631-42.
Meghri NW, Donius AE, Riblett BW, Martin EJ, Morss Clyne A, and Wegst UGK. (2010) Directionally Solidified Biopolymer Scaffolds: Mechanical Properties and Endothelial Cell Responses. JOM 62 (7): p. 71-5.
Kalghatgi S, Friedman G, Fridman A, Morss Clyne A. (2010) Low dose non-thermal plasma treatment enhances endothelial cell proliferation through FGF-2 release. Annals of Biomedical Engineering 38 (3): p. 748-57.
Buyukhatipoglu K, Morss Clyne A. (2010) Controlled flame synthesis of αFe2O3 and Fe3O4 nanoparticles: effect of flame configuration, flame temperature and additive loading. Journal of Nanoparticle Research 12 (4): p. 1495-1508.
Buyukhatipoglu K, Jo W, Sun W, Morss Clyne A. (2009) The role of printing parameters and scaffold biopolymer properties in the efficacy of a new hybrid nano-bioprinting system. Biofabrication 1 (3): 035003.
Morss Clyne A, Edelman, ER. (2009) Vascular growth factor binding kinetics to the endothelial cell basement membrane, with a kinetics-based correction for substrate binding. Cytotechnology 60 (1): p. 33-44.
Buyukhatipoglu K, Miller TA, Morss Clyne A. (2009) Interaction of superparamagnetic, flame synthesized iron oxide nanoparticles with porcine aortic endothelial cells: toxicity, cellular uptake and proliferation studies. Journal of Nanoscience and Nanotechnology 9 (12): p. 6834-6843.
Morss Clyne, AS, Zhu, H, Edelman, ER. (2008) Elevated fibroblast growth factor-2 increases tumor necrosis factor-α induced endothelial cell death in high glucose. Journal of Cellular Physiology. 217: p. 86–92.
Morss AS, Edelman ER (2007). Glucose modulates basement membrane absorption of fibroblast growth factor-2 via alterations in endothelial cell permeability. Journal of Biological Chemistry. 282(19): p. 14635-14644.
Edelman ER, Seifert P, Groothuis A, Morss A, Bornstein D, Rogers C (2001). Gold-coated NIR stents in porcine coronary arteries. Circulation. 103(3): p. 429-434.
BOOK CHAPTERS
Urbano R, Morss Clyne A. Cell-substrate interactions. In Molecular and Cellular Biomechanics. Ed. Bradley Layton, Pan Stanford Publishing
Morss Clyne A. Reactive oxygen species in physiologic and pathologic angiogenesis. In Mechanical and Chemical Signaling in Angiogenesis. Ed. Cynthia Reinhart-King, Springer- Verlag Publishing
CONFERENCE PRESENTATIONS (* undergraduate)
Canver A, Urbano R, Morss Clyne A. The impact of matrix glycation and mechanics on endothelial cell mechanotransduction, collective migration, and inflammatory response (Invited talk) 2014 World Congress of Biomechanics
Morss Clyne A. Engineering the Mechanobiology Bridge: from Cells to Communities (Invited talk) 2014 International Workshop on Multiscale Mechanobiology