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Zeiss AxioObserver Z1- Wide-field microscope

The Zeiss AxioObserver is an inverted microscope - objectives are below the stage. The microscope is ideal for 2D or 3D imaging of thin specimens (one-cell layer).

Light Sources

Halogen lamp for bright-field microscopy

  • a contrast image of the specimen is obtained under white light illumination

Metal halide arc lamp for fluorescence microscopy 

  • excitation and emission filters are available for imaging fluorescent probes

Excitation filters (nm)

 Emission filters (nm)

Corresponding fluorescent probes 

 340-390  420-470  DAPI
 450-490  500-550  GFP / YFP / FITC / Alexa 488 / Cy2
 530-460  590-650  tdTomato / mCherry / TRITC / Cy3
 625-655  665-715  Alexa 647 / Cy5


(Magnification / Numerical aperture) 

  • 10x/0.3 Dry (Zeiss)
  • 20x/0.4 Dry and 20x/0.8 Dry (Zeiss)
  • 63x/1.4 Oil (Zeiss)
  • 100x/1.4 Oil (Zeiss)


Hamamatsu Orca-R2 (CCD camera)

Acquisition software

Slidebook 6 with 2D and 3D deconvolution module