For a better experience, click the Compatibility Mode icon above to turn off Compatibility Mode, which is only for viewing older websites.

Zeiss AxioObserver Z1- Wide-field microscope

The Zeiss AxioObserver is an inverted microscope - objectives are below the stage. The microscope is ideal for 2D or 3D imaging of thin specimens (one-cell layer).

https://cdnapisec.kaltura.com/p/1513041/sp/151304100/embedIframeJs/uiconf_id/43882971/partner_id/1513041?iframeembed=true&playerId=kaltura_player&entry_id=1_5fewdkco&flashvars[localizationCode]=en&flashvars[sideBarContainer.plugin]=true&flashvars[sideBarContainer.position]=left&flashvars[sideBarContainer.clickToClose]=true&flashvars[chapters.plugin]=true&flashvars[chapters.layout]=vertical&flashvars[chapters.thumbnailRotator]=false&flashvars[streamSelector.plugin]=true&flashvars[EmbedPlayer.SpinnerTarget]=videoHolder&flashvars[dualScreen.plugin]=true&flashvars[hotspots.plugin]=1&flashvars[Kaltura.addCrossoriginToIframe]=true&&wid=1_h75rznmn

Light Sources

Halogen lamp for bright-field microscopy

  • a contrast image of the specimen is obtained under white light illumination

Metal halide arc lamp for fluorescence microscopy 

  • excitation and emission filters are available for imaging fluorescent probes

Excitation filters (nm)

 Emission filters (nm)

Corresponding fluorescent probes 
 340-390  420-470  DAPI
 450-490  500-550  GFP / YFP / FITC / Alexa 488 / Cy2
 530-460  590-650  tdTomato / mCherry / TRITC / Cy3
 625-655  665-715  Alexa 647 / Cy5

Objectives

(Magnification / Numerical aperture) 

  • 10x/0.3 Dry (Zeiss)
  • 20x/0.4 Dry and 20x/0.8 Dry (Zeiss)
  • 63x/1.4 Oil (Zeiss)
  • 100x/1.4 Oil (Zeiss)

Detector

Hamamatsu Orca-R2 (CCD camera)

Acquisition software

Slidebook 6 with 2D and 3D deconvolution module