Olympus-IX81- Wide-field microscope
The Olympus IX81 is a fully automated inverted microscope - objectives are below the stage. This microscope is ideal for 2D and 3D imaging of fixed and live specimens. Excitation and emission filter wheels enable fast time-lapse imaging. The microscope is equipped with a temperature-controlled chamber for imaging living cells.
A stereology application is available on the IX81. Stereological techniques are performed to estimate the total number, volume, and surface area of objects in a biological structure. For instance, stereology uses random and unbiased sampling of tissue sections to extrapolate the total number of cells in a 3D structure.
A color camera is also available for histochemical and immunohistochemical staining.
Halogen lamp for bright-field microscopy
- a contrast image of the specimen is obtained under the white light illumination
Mercury arc lamp for fluorescence microscopy
- excitation and emission filters are available for imaging different fluorescent probes
Excitation filters (nm)
Emission filters (nm)
Corresponding fluorescent probes
|| GFP / YFP / FITC / Alexa 488 / Cy2
|| tdTomato / mCherry / TRITC / Cy3
|| Alexa 647 / Cy5
|| 467-599 / 528-556
|| CFP / YFP FRET pair
(Magnification / Numerical aperture)
- 20x/0.7 Dry (Olympus)
- 60x/1.4 Oil (Olympus)
- 100x/1.4 Oil (Olympus)
Hamamatsu Orca-Flash4.0 (sCMOS camera)
Lumenera Infinity 1-3C (color CMOS camera)
Slidebook 6 with 2D and 3D deconvolution module, and stereology module