Zeiss AxioObserver Z1- Wide-field microscope
The Zeiss AxioObserver is an inverted microscope - objectives are below the stage. The microscope is ideal for 2D or 3D imaging of thin specimens (one-cell layer).
Light Sources
Halogen lamp for bright-field microscopy
- a contrast image of the specimen is obtained under white light illumination
Metal halide arc lamp for fluorescence microscopy
- excitation and emission filters are available for imaging fluorescent probes
| Excitation filters (nm) | Emission filters (nm) | Corresponding fluorescent probes |
|---|---|---|
| 340-390 | 420-470 | DAPI |
| 450-490 | 500-550 | GFP / YFP / FITC / Alexa 488 / Cy2 |
| 530-460 | 590-650 | tdTomato / mCherry / TRITC / Cy3 |
| 625-655 | 665-715 | Alexa 647 / Cy5 |
Objectives
(Magnification / Numerical aperture)
- 10x/0.3 Dry (Zeiss)
- 20x/0.4 Dry and 20x/0.8 Dry (Zeiss)
- 63x/1.4 Oil (Zeiss)
- 100x/1.4 Oil (Zeiss)
Detector
Hamamatsu Orca-R2 (CCD camera)
Acquisition software
Slidebook 6 with 2D and 3D deconvolution module
