Zeiss AxioObserver Z1- Wide-field microscope

The Zeiss AxioObserver is an inverted microscope - objectives are below the stage. The microscope is ideal for 2D or 3D imaging of thin specimens (one-cell layer).

Zeiss Video

Light Sources

Halogen lamp for bright-field microscopy

  • a contrast image of the specimen is obtained under white light illumination

Metal halide arc lamp for fluorescence microscopy 

  • excitation and emission filters are available for imaging fluorescent probes
Excitation and Emission Filters
Excitation filters (nm) Emission filters (nm) Corresponding fluorescent probes
340-390 420-470 DAPI
450-490 500-550 GFP / YFP / FITC / Alexa 488 / Cy2
530-460 590-650 tdTomato / mCherry / TRITC / Cy3
625-655 665-715 Alexa 647 / Cy5
Zeiss AxioObserver widefield epifluroscence microscope

Objectives

(Magnification / Numerical aperture) 

  • 10x/0.3 Dry (Zeiss)
  • 20x/0.4 Dry and 20x/0.8 Dry (Zeiss)
  • 63x/1.4 Oil (Zeiss)
  • 100x/1.4 Oil (Zeiss)

Detector

Hamamatsu Orca-R2 (CCD camera)

Acquisition software

Slidebook 6 with 2D and 3D deconvolution module 

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Location

Papdakis Integrated Sciences Building
Cell Imaging Center
Drexel University
Papadakis Integrated Sciences Building
4th Floor
3245 Chestnut Street
Philadelphia, PA 19104

Location