Electrospun Scaffolds for Spinal Cord Explant Cultures

Wednesday, May 30, 2018

2:00 PM-4:00 PM

BIOMED PhD Thesis Defense

Title:
Electrospun Scaffolds for Spinal Cord Explant Cultures

Speaker:
Reva M. Street, PhD Candidate, School of Biomedical Engineering, Science and Health Systems, Drexel University

Advisor:
Caroline L. Schauer, PhD, Associate Professor, Department of Materials Science and Engineering, Drexel University

Abstract:
In vitro study of spinal cord explants is a valuable model for research into strategies for improving neural interfaces and studying neuronal biology, for example the formation of the neuromuscular junction, neuronal survival and dendrite outgrowth. This is especially true as in vivo studies are challenging due to technical limitations and a high level of complexity. Electrospun fibers are an excellent interface material, as they can not only serve as a topographically tunable culture substrate, but can also be used for controlled delivery of neuroprotective molecules in vitro.

In this thesis, the formulation of electrospun nanofibers for enhanced in vitro neuronal outgrowth is explored using three approaches: (1) The alteration of nanofiber surface chemistry through the addition of cationic polyethylenimine. (2) The use of electrospun piezoelectric polymers to induce electrical stimulation by transient surface charges. (3) The release of neuroprotective biomolecule uric acid.

Through these three approaches, an electrospun platform with improved neuronal biocompatibility for the study of in vitro spinal cord explants has been achieved.

Contact Information

Ken Barbee
215-895-1335
barbee@drexel.edu