Microscopy Techniques

Main Techniques

Köhler illumination 

• Description
• Tutorial

Differential interference contrast

• Description
• Tutorial

Deconvolution

• Description 

Confocal microscopy

• Concepts in Confocal Microscopy
• Confocal Microscopy - Optical Path 

Super-resolution

General concepts

• Introduction to Superresolution Microscopy
• Super-resolution molecular and functional imaging of nanoscale architectures in life and materials science, Habuchi S, 2014
• Recent advances in super-resolution fluorescence imaging and its applications in biology, Han R et al., 2013
• Fluorescence nanoscopy. Methods and applications. Requejo-Isidro J, 2013
• Review of super-resolution fluorescence microscopy for biology. Leung BO & Chou KC, 211
• Super-resolution microscopy at a glance, Galbraith CG & Galbraith JA, 2011|
• A new wave of cellular imaging. Toomre D & Bewersdorf J, 2010
• A guide to super-resolution fluorescence microscopy. Schermelley L et al., 2010
• Super-resolution fluorescence microscopy, Huang B et al., 2009
  

Localization microscopy

• Introduction to photoactivated Localization Microscopy
• Practical Aspects of PALM Imaging
• Localization microscopy coming of age: from concepts to biological impact. Sauer M, 2013
• Super-resolution fluorescence imaging with single molecules. Sahl SJ, Moerner WE, 2013 

SIM (Structured Illumination Microscopy)

• Superresolution Structured Illumination Microscopy
• Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination, Fiolka R et al., 2012
• Three-dimensional structured illumination microscopy and its application to chromosome structure, Carlton PM, 2008 

STED (Stimulated Emission Depletion)

• STED microscopy - towards broadened use and scope of applications, Blom H & Widengren J, 2014
• STED microscopy of living cells -- new frontiers in membrane and neurobiology, Eggeling C et al., 2013
• 3D STED (Stimulated Emission Depletion) Microscopy