Research in the Department of Biology has grown in leaps and bounds over the past decade. The department has procured a number of research assets, including comprehensive and fully-maintained research centers.
The Cell Imaging Center is a state-of-the-art light microscopy facility that serves the imaging needs of Biology labs and researchers from a variety of academic departments and colleges. The facility operates on a fee-for-service basis and instrumentation is available to registered and trained users through a web-based reservation system. Users are trained to independence, but facility-run sessions can be arranged for users with short term imaging needs. The CIC is located in the Papadakis Integrated Sciences Building and includes the following instrumentation
- Olympus FluoView 1000 laser scanning confocal microscope
- Zeiss LSM 700 laser scanning confocal microscope
- GE Healthcare (Applied Precision) DeltaVision OMX V4 super-resolution (3D structured illumination and single molecule localization) microscope
- Bruker (Prairie Technologies) two-photon laser scanning microscope
- Zeiss AxioObserver 2 wide-field fluorescence microscope
- Olympus IX81 wide-field fluorescence microscope
Location: PISB 408/308
As a joint effort amongst the faculty, this state of the art piece of equipment provides a vast array of experimentation for your immunology needs. Here, we have the BD Biosciences FACS CANTO I, capable of cell sorting and multiparametric analysis.
Location: PISB 411
The RNAi Resource Center, hosted by the Department of Biology at Drexel Univeristy provides HUMAN shRNA from the Open Biosystems Human Expression Arrest©™ shRNA library. We bring you the freedom and use of RNAi technologies at a HUGE discount. Here, you can easily order any construct from the entire Human Expression Arrest©™ shRNA library. As well as supplying the reagents, we are here to help facilitate the implementation of RNAi within your lab.
Location: PISB 401
An AKTA FPLC™ system is available for the separation and characterization of biomolecules and is especially suited for the purification of proteins. Fast-performance liquid chromatography can be utilized to characterize the hydrodynamic behavior of biomolecules over a large size range (1 > 1,000,000 daltons) and can be implemented in analytical and preparative scales.
The AKTA FPLC™ system is equipped with UV and conductivity detectors and an automated fraction collector. The system is controlled via UNICORN software and a Method Wizard can be used for easy design of purification schemes. A wide variety of chromatography media is available for separation by size exclusion, ion exchange, hyrdrophobicity or affinity chromatography. Instruments available to researchers include the Agilent 6890 Gas Liquid Chromatograph with capillary inlet system and flame ionization detector and autosampler and the Agilent Series 1100 High Performance Liquid Chromatograph with autosampler, quaternary pumps, thermostated column chamber, fluorescence detector and diode array detector. Both instruments are connected to an HP Kyak workstation running Agilent ChemStation software for instrument control, data acquisition and data evaluation.
Location: PISB 401
The FluorChem-8900 Chemiluminescence and Gel Imager from Alpha Innotech can be used for a wide array of quantitative imaging applications, from chemiluminescence imaging, fluorescence imaging, gel, film and membrane imaging to culture and microplate-based assays.
The equipment has an excellent image quality combined with easy-to-use software features. It has a CCD camera with 1.92 Megapixel Resolution with a 12-bit Dynamic Range ideal for chemiluminescence, fluorescence and absorbance imaging. The analysis software can perform a broad range of analysis likes Molecular Weight calculation, Rf determination, 1-D lane densitometry, 2-D spot, quantitative PCR, gel scoring and automated colony counting. The software comes with Movie and Auto Exposure modes.
Location: PISB 405
The Perkin Elmer TopCount NXT microplate scintillation and luminescence counter is a fast, convenient, and versatile instrument for detecting beta and gamma emitting isotopes such as 3H, 125I, 14C, 35S, 45Ca, 51Cr, 33P and 32P. TopCount differentiates true scintillation events from background and allows the researcher to count events directly in one or multiple standard size microtiter plates ranging in size from 24 to 384 wells. Researchers in the Department of Biology are currently using this equipment to monitor enzymatic activity, such as luciferase luminescence, and cellular proliferation and activities, such as the clonal expansion of radioactively-labeled lymphocytes in response to antigenic stimulation.
Location: PISB 401
To quantify the amount of radioactivity in samples commonly generated during biochemical assays involving radiolabeled substrates, the department has a liquid scintillation counter. It is a Packard Tricarb 1500 model. This machine is also used for measuring swipe tests to allow adherence to regulations regarding safe use of radioactivity in laboratories.
Location: PISB 405 / 412
Agilent 6890 Gas Liquid Chromatograph with capillary inlet system and flame ionization detector and autosampler. Agilent Series 1100 High Performance Liquid Chromatograph with autosampler, quaternary pumps, thermostated column chamber, fluorescence detector and diode array detector.
Both are connected to an HP Kyak workstation running Agilent ChemStation software for instrument control, data acquisition and data evaluation.
Location: PISB 307