Illumina NextSeq 500

• Bench top: slots in between MiSeq and HiSeq
• 120 gigabases of sequence; 40x coverage of human genome/run
• Expected upgrades over next year or so should take in to 3 human genomes/run
• 12 and 30 hour cycle times
• Short reads: 1x75 bases; 2x75 bases; 2x150 bases
• Has two flow cell configurations – mid (132M clusters) and high (400M clusters)
  • 1-16 exomes/run
  • 1-20 transcriptomes/run
  • 6-96 targeted panels/run
  • 12-40 gene expression profiling samples/run

Illumina MiSeq

• Up to 15 gigabases of output
• Read Length: 1x36 bp, 2x25 bp, 2x75 bp, 2x150 bp, 2x250 bp, 2x300 bp
• ~4 to ~56 hour run time
• 4 M to 44-50 M clusters

Pacific Biosciences Sequel System (PacBio)

• 1 million ZMWs per SMRTcell
• Longest average read lengths
• Highest consensus accuracy
• Uniform coverage
• Simultaneous epigenetic characterization
• Single-molecule resolution

Pacific Biosciences Sequel System IIe (PacBio)

Based on the proven performance of the Sequel II system, the Sequel IIe system delivers:

• Direct access to the only highly accurate long reads: PacBio HiFi reads
• Deeper biological insights, less data processing, and faster results thanks to the unmatched clarity of HiFi reads
• Reliable and affordable high-throughput sequencing for a broad range of applications
• 8 million ZMWs per SMRTcell

Key benefits of HiFi sequencing on the Sequel IIe system:

• Better data for superior results with lower coverage, from a single technology
• Less time spent on data processing and analysis for faster answers
• Cost savings at every step of your sequencing pipeline

Bio-Rad QX-200 Droplet Digital PCR

• Absolute quantitative limiting dilution PCR for DNA or RNA – based on Poisson distribution
• Analogous to 454 emulsion technology – partitions ddPCR mix into 20K nL droplets (one template max/droplet)
• EvaGreen or Taqman-type hydrolysis probe-based
  • Droplet digital PCR instruments
    • C1000 touch thermal cycler – performed on partitioned droplets
    • PX1 plate sealer
  • Droplet generator
  • Droplet reader – droplets are sipped and run single file past a two-color detector
    • # of positive and negative droplets is counted to give discrete quantitative data
• No need for standard curves
  • NGS library quantitation for multiplex strategies
  • Gene expression studies
  • Copy number variations
  • Viral load
  • Cancer biomarkers

BluePippin

• DNA size selection for next-gen sequencing, with pulsed field
• 100bp – 50kb
• Collect HMW DNA with high-pass filtering

Femto Pulse System

• Large fragment resolution – separate high molecular weight DNA smears and fragments through 165 kb
• Achieves 10 times higher sensitivity for nucleic acid smears and up to 100 times higher for nucleic acid fragments
• Detects nucleic acids down to 50 fg/µL input concentration, making the Femto Pulse System ideal for long-read NGS QC, gDNA, small RNA, or cfDNA analysis from low-concentration samples

2100 Bioanalyzer Instrument

• Objective assessment of sizing, quantitation, integrity and purity from DNA, RNA and proteins
• Minimal sample volumes are required for an accurate result

Qubit 4 Fluorometer

• Latest version of the popular Qubit fluorometer
• Accurately measure DNA, RNA and protein quantity
• RNA integrity and quality
• High levels of accuracy using only 1–20 μL of sample

nanoString

• nCounter analysis system – for targeted quantitative nucleic acid analyses
• Highly multiplexable – can look at up to 800 targets simultaneously
  • Digital – no amplification, uses nanotech colored barcodes
    • Each target unique bar code
  • Sensitive – more sensitive than PCR-based systems
  • DNA (CNV), mRNA, miRNA, lncRNA, ChIP-string analysis
  • Very small sample sizes
    • In vivo studies of pathogen gene expression
    • Single cell expression
• Workflow
  • Set up hybridization between target and nCounter probes
  • Hybridize and wash ON on robotic prep station
  • Move hybridization cartridge to digital analyzer
  • Unparalleled sensitivity (< 1 copy/cell), reproducibility (R2 > .99), and dynamic range (5 logs)