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He Lin, PhD

Lin He, PhD

Visiting Professor
Department of Chemistry
Phone: 919.515.2993


  • PhD, Chemistry, The Pennsylvania State University

Research Interests:

Significant advances in modern technology have enabled scientists to explore and comprehend nature at a level of detail never dreamed of. To continue this fast-paced progress, the development of novel strategic methodologies to address complex scientific questions is imperative. Equipped with interdisciplinary approaches, my research program is centered on developing novel analytical tools and sensors and exploiting their applications in the field of bioanalytical measurements. In particular, we have been working on coupling the novel properties of various nanomaterials with conventional analytical techniques to develop:

  1. a new sensing modality based on amplification-by-polymerization for POC DNA diagnostics
  2. a label-free multiplexed biomolecule detection platform based on a hybrid of encoded nanoparticles and conjugated fluorescent polymers
  3. a high resolution single particle imaging tool based on plasmon-enhanced circular dichroism
  4. nanofeatures-facilitated mass spectrometric techniques in tissue imaging and metabolite profiling

The overarching aims of our research is to introduce new means to monitor elementary biomolecular interactions with possible ramifications in field-friendly biomolecule screening and biomolecular-facilitated photocatalytic reactions.


  • Deputy Acting Director, Chemistry Division, NSF, 2018-present
  • Program Director, Chemistry Division, NSF, 2016-2018
  • Adjunct Associate Professor, Department of Chemistry, NC State University, 2016-present
  • Rotating Program Director, Chemistry Division, NSF, 2011-2015
  • Adjunct Professor, Department of Biomedical Engineering, UNC/NCSU, 2016-2016
  • Associate Professor, Department of Chemistry, North Carolina State University, 2016-2016
  • Honorary Professor, Department of Chemistry, Southeast University, PR China, 2007-2016
  • Assistant Professor, Department of Chemistry, North Carolina State University, 2003-2009
  • Senior Scientist/Manager, Nanoplex Technologies, Inc., Mountain View, CA, 2002-2003

Selected Publications:

  • Zhan, K.; He, L. "Single Particle Imaging of Surface-Plasmon Enhanced CircularDichroism", Submitted.
  • Moening, T. N.; Brown V. L.; He, L. "Metrix-Enhanced Nanostructure Initiator MassSpectrometry (ME-NIMS) for Mass Spectrometry Imaging", Anal. Method. 2016, 8, 8234-8240.
  • Moening, T. N.; Brown V. L.; He, L. "Nanostructure-Initiator Mass Spectrometry (NIMS)for Molecular Mapping of Animal Tissues" book chapter in "Mass Spectrometric Imaging ofSmall Molecules", Methods in Molecular Biology Series, He. L, Eds. Humana Press 2014.
  • Brown V. L.; Liu, Q.; He, L. "Matrix-Enhanced Surface-Assisted LaserDesorption/Ionization Mass Spectrometry (ME-SALDI-MS) for Mass Spectrometry Imagingof Small Molecules " book chapter in "Mass Spectrometric Imaging of Small Molecules",Methods in Molecular Biology Series, He. L, Eds. Humana Press 2014.
  • Zheng, W.; He, L. "Thermodynamic and Kinetic Study of Formation of Cationic ConjugatedPolymer-DNA Complexes", Bioscience Mater. 2014, 2, 1471-1479.
  • Zheng, W.; Chase, T. E.; He, L. "Label-free Multiplexing Detection of miRNA UsingConjugated Polymers and Striped Nanoparticles", Anal Method, 2014, 6, 2399-2405.
  • Liu, Q.; Brown, V. L.; He, L. "Desorption/Ionization on Porous Silicon (DIOS) forMetabolite Imaging" Invited chapter for inclusion in "Porous Silicon for Biomedical Applications", Woodhead Publishing Series in Biomaterials 68#, H. A. Santos, Eds. Woodhead Publishing 2013. pg 270-285.
  • Zheng, W.; He, L. "Multiplexed Immunoassays", Invited chapter in MultiplexedImmunoassays, Advances in Immunoassay Technology, Norman H. L. Chiu (Ed.), InTech,2012.
  • He, P.; Tucker, E. Z.; Gorman, C. B.; He, L "Chemical Amplification for In-Gel DNADetection" Anal. Method. 2011, 3, 2463-2468.
  • Wu Y.; Liu S.; He L. "Polymerization-assisted signal amplification for electrochemicaldetection of biomarkers" Analyst 2011, 136, 2558-2563.
  • Wu, Y.; Liu, S.; He, L. "Activators generated electron transfer for atom transfer radicalpolymerization for immunosensing”, Biosensor Bioelectron. 2010, 26, 970-975.
  • Zheng, W.; He, L. "Distance-dependent fluorescence quenching of conjugated polymer on Au/Ag striped nanorods", J. Phys. Chem. C, 2010, 114, 17829–17835.
  • Qian, H. He, L. "Polymeric Macroinitiators for Signal Amplification in AGET ATRP-based DNA Detection", Sens. Actuator B, 2010, 150, 594-600.
  • Liu, Q.; Xiao, Y.; He, L. "Mass Spectrometry Imaging of Small Molecules Using Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS)" Invited chapter for inclusion in "Mass Spectrometric Imaging: History, Fundamentals and Protocols", Methods in Molecular Biology Series, Jonathan Sweedler and Stanislav Rubakhin, Eds. Humana Press 2010.
  • Zheng, W.; He, L. "Multiplexed detection of protein cancer markers on Au/Ag-barcoded nanorods using fluorescent-conjugated polymers" Anal. Bioanal. Chem 2010, 397, 2261–2270.
  • Qian, H.; He, L. "Detection of Protein Binding Using Polymerization-Assisted Amplification" Anal Chem, 2009, 81, 9824–9827.
  • Liu, Q.; He, L. "Ionic Matrix for Matrix-Enhanced Surface-Assisted Laser Desorption Ionization Imaging Mass Spectrometry (ME-SALDI-IMS)” J. Am. Soc. Mass Spectrom. 2009, 20, 2229-2237.
  • Wu, Y.; Liu, S.; He, L. "Electrochemical Biosensing Using Amplification-by-Polymerization”, Anal. Chem. 2009, 81, 7015–7021.
  • Qian, H. He, L. "Surface-initiated AGET ATRP for Detection of DNA Point Mutation” Anal Chem. 2009, 81, 4536–4542.
  • He, P.; He, L. "Synthesis of Surface-Anchored DNA-Polymer Bioconjugates Using Reversible Addition-Fragmentation Chain Transfer Polymerization (RAFT)”, Biomacromolecules, 2009, 10, 1804–1809.
  • Zheng, W.; He, L. “Label-Free, Real-Time Multiplexed DNA Detection Using Fluorescent Conjugated Polymers” J. Am. Chem. Soc. 2009, 131, 3432–3433.
  • Zheng, W.; He, L. “Particle Stability In Polymer-Assisted Reverse Colorimetric DNA Assays” Anal Bioanal Chem 2009, 393, 1305–1313.
  • Xiao, Y.; Retter, S. T.; Thomas, D. K.; He, L. “Impacts of Surface Morphology on Ion Desorption and Ionization in Desorption Ionization on Porous Silicon (DIOS) Mass Spectrometry”, J. Phys. Chem. C. 2009, 113, 3076–3083.
  • Liu, Q.; Xiao, Y.; Pagan-Miranda, C.; Chiu, Y. M.; He, L. “Metabolite Imaging Using Matrix-Enhanced Surface-Assisted Laser Desorption Ionization Mass Spectrometry (ME-SALDI-MS)” J. Am. Soc. Mass Spectrom. 2009, 20, 80-88.
  • He, P.; Zheng, W.; Tucker, E. Z.; Gorman, C. B.; He, L. “Reversible Addition-Fragmentation Chain Transfer (RAFT) Polymerization in DNA Biosensing”, Anal. Chem. 2008, 80, 3633-3639.
  • Lou, X.; He, L. “Surface Passivation Using Oligo(Ethylene Glycol) in ATRP-Assisted DNA Detection”, Sens. Actuator B, 2008, 129, 225–230.
  • Liu, Q.; He, L. “Quantitative Study of Solvent and Surface Effects on Analyte Ionization in Desorption Ionization on Silicon (DIOS) Mass Spectrometry”, J. Am. Soc. Mass Spectrom. 2008, 19, 8-13.
  • Wu, Y.; Zhuang, Y.; Liu, S.; He, L. "Phenylboronic acid immunoaffinity reactor coupled with flow injection chemiluminescence for determination of α-fetoprotein", Anal. Chim. Acta 2008, 630, 186-193.
  • Liu, S.; Peng, L.; Yang, X. D.; Wu, Y. F.; He, L. " Electrochemistry of cytochrome P450 enzyme on nanoparticle-containing membrane-coated electrode and its applications for drug sensing", Anal. Biochem. 2008, 375, 209-216.
  • Liu, S.; Zhang, X.; Wu, Y.; Tu, Y.; He, L. "Prostate-Specific Antigen Detection by Using a Reusable Amperometric Immunosensor Based on Reversible Binding and Leasing of HRP-Anti-PSA from Phenylboronic Acid Modified Electrode", Clin. Chim. Acta 2008, 395, 51-56.
  • Okelo, G. O.; He, L. “Cu(0) as the Reaction Additive for Purge-Free ATRP-Assisted DNA Detection”, Biosensor Bioelectron. 2007, 23, 588–592.
  • Liu, S.; Wang, K.; Du, D.; He, L. “Recognition of Glycoprotein Peroxidase via Con A-Carrying Self-Assembly Layer on Gold”, Biomacromolecules, 2007, 8; 2142-2148.
  • Lou, X.; Wang, C.; He, L. “Core-shell Au Nanoparticle Formation with DNA-Polymer Hybrid Coatings Using Aqueous ATRP”, Biomacromolecules, 2007, 8, 1385-1390.
  • Liu, Q.; Guo, Z. He, L. "Mass Spectrometry Imaging of Small Molecules Using Desorption/Ionization on Silicon (DIOS)", Anal. Chem. 2007, 79, 3535-3541.
  • Guo, Z.; He, L. "A Binary Matrix for Background Suppression in MALDI-MS of Small Molecules", Anal. Bioanal. Chem. 2007, 387, 1939-1944.
  • Nordstrom A., He, L., Siuzdak G. "Desorption/Ionization on Silicon (DIOS)" Encyclopedia of Mass Spectrometry, Vol 6, Ionization Method, Editor Michael Gross, 2007, 6, 676-683.
  • Liu, Q.; Xiao, Y.; He, L. “Book Review: MALDI MS: A Practical Guide to Instrumentation, Methods and Applications” ChemBioChem 2007, 8, 2315-2316.
  • Lou, X.; He, P.; Okelo, G. O.; He, L. "Radical Polymerization in Biosensing" Anal. Bioanal.Chem 2006, 384, 525-531.
  • Lou, X.; He, L. "DNA-Accelerated Atom Transfer Radical Polymerization (ATRP) on a AuSurface" Langmuir, 2006 22, 2640-2646.
  • Guo, Z.; Ganawi, A. A. A.; Liu, Q.; He, L. "Nanomaterials in Mass SpectrometricMeasurements and its Perspectives in Bioanalysis" Anal. Bioanal. Chem. 2006, 384, 584-592.
  • Lou, X.; Lewis, M. S.; Gorman, C. B., He, L. "Detection of DNA Point Mutation by AtomTransfer Radical Polymerization (ATRP)" Anal. Chem. 2005, 77, 4698-4705.
  • Finkel, N. H.; Prevo, B. G.; Velev, O. D.; He, L. "Ordered Silicon Nanocavity Arrays inSurface-Assisted Desorption/Ionization Mass Spectrometry" Anal. Chem. 2005, 77, 1088-1095.
  • Freeman, R.G.; Raju, P.; Norton, S.; Walton, I.; Smith, P.; He, L.; Natan, M. J.; Sha, M.;Penn, S. G. "Use of Nanobarcodes Particles in Bioassays" Methods Mol. Biol. 2005, 303, 73-83.
  • Lou, X.; He, L. "Synthesis of DNA-Polymer Hybrids Using Aqueous Solution-Based AtomTransfer Radical Polymerization (ATRP)" Polymer Preprint 2004, 45, 455-456.
  • Finkel, N. H; Lou, X.; Wang, C.; He, L. "Barcoding the Microworld" Anal. Chem. 2004, 76,352A-359A.
  • Penn, S. G.; He, L.; Natan, M. J. "Nanoparticles for Bioanalysis" Expected Mater. Future,2005, 5, 8-16 (Japanese Translation of Curr. Opin. Chem. Biol.).
  • He, L.; Smith, E. A.; Natan, M. J.; Keating, C. D. "The Distance Dependence of ColloidalAu-Amplified Surface Plasmon Resonance " J. Phys. Chem. B. 2004, 108, 10973-10980.
  • Penn, S. G.; He, L.; Natan, M. J. "Nanoparticles for Bioanalysis" Curr. Opin. Chem. Biol.2003, 7, 609-615.
  • Mulvaney, S. P.; He, L.; Natan, M. J.; Keating, C. D. "Three-Layer Substrates for SurfaceEnhanced Raman Scattering: Preparation and Preliminary Evaluation" J. Raman Spectrosc.2003, 34, 163-171.
  • Walton, I. D.; Norton, S. M.; Balasingham, A.; He, L.; Oviso Jr., D. F.; Gupta, D.; Raju, P.A.; Natan, M. J.; Freeman, R. G. "Particles For Highly Multiplexed Analysis In Solution:Detection And Identification Of Striped Metallic Particles Using Optical Microscopy" Anal.Chem. 2002, 74, 2240-2247.
  • Nicewarner-Pena, S. R.; Freeman, R. G.; Reiss, B, D.; He, L.; Pena, D. J.; Walton, I. D.;Cromer, R.; Keating, C. D.; Natan, M. J. "Submicrometer Metallic Barcodes" Science 2001,294, 137-141.
  • Goodrich, G. P.; Nicewarner, S. R.; He, L.; Natan, M. J.; Keating, C. D. "Nanoparticle-Amplified Surface Plasmon Resonance For Detection Of DNA Hybridization" Proc. SPIE-Int. Soc. Opt. Eng. 2001, 4258, 80-85.
  • Wang, D.; He, L.; Freeman, R. G.; Cromer, R.; Natan, M. J.; Singh, R. "NanobarcodesIdentification Tags In Proteomics and Small Molecule Profiling" Proc. μTas 2001 Symp.2001, 197-198.
  • He, L.; Musick, M. D.; Nicewarner, S. R.; Salinas, F. G.; Benkovic, S. J.; Natan, M. J.;Keating, C. D. "Colloidal Au-Enhanced Surface Plasmon Resonance for UltrasensitiveDetection of DNA Hybridization" J. Am. Chem. Soc. 2000, 122, 9071-9077.
  • He, L.; Natan, M. J.; Keating, C. D. "Surface-Enhanced Raman Scattering: A Structure-Specific Detection Method For Capillary Electrophoresis" Anal. Chem. 2000, 72, 5348-5355.
  • He, L.; Mulvaney, S. P.; St. Angelo, S. K.; Baker, B. E.; Natan, M. J. "EnvironmentalApplication of Highly Enhancing Substrates for Surface Enhanced Raman Scattering(SERS)" ACS Symp. Ser. 2000, 761, 366-373.
  • Lyon, L. A.; Keating, C. D.; Fox, A. P.; Baker, B. E.; He, L.; Nicewarner, S. R.; Mulvaney,