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Location

Disque Hall Room 919

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Physics Colloquium: "Using Repeat Proteins to Probe Stability, Cooperativity, and Pathway Selection"

Thursday, February 14, 2013

3:30 PM-4:30 PM


Speaker: Dr. Doug Barrick, Johns Hopkins University
 
Abstract: One of the most striking features of the protein folding process is cooperativity. Although the structures of the native states of proteins comprise many short- and long-range elements that might appear to be independent (in a thermodynamic sense), these elements are in fact highly coupled, populating not as independent subsets but as a whole. Cooperativity in folding is likely to use the same underlying interactions, and thus, have the same thermodynamic features as functional properties such as allostery and assembly. Despite the importance of cooperativity in folding, our analysis of it remains largely at the "yes" or "no" (i.e., two-state versus multistate) level.

We have been using linear repeat proteins to extend our description of cooperativity in folding to a quantitative (thermodynamic) level. By constructing an array of 33-residue ankyrin repeats that have varying length but identical (consensus) sequence, we have resolved the high cooperativity of unfolding into local versus nearest-neighbor energy, entropy, and heat capacity. This approach has revealed an extremely strong coupling free energy between repeats, consistent with results from the Pluckthun lab; we find this coupling to be entropically driven at low temperatures, consistent with hydrophobic desolvation. This high level of coupling is considerably stronger than that seen in a set of consensus TPR repeats studied by the Regan lab. A high level of cooperativity can also be achieved with consensus LRR  Beta-sheet repeat proteins, although we find that design well-behaved consensus LRRs is a considerably greater challenge than for helical arrays.

Linear repeat proteins are also well-suited for kinetic studies of folding. Due to the high level of structural symmetry of naturally-occurring (sequence-variable) repeat proteins, and the additional sequence symmetry of consensus repeat counterparts, we can examine the effects of chain-length on folding rates, the presence of parallel pathways, and the relationship between stability variation and cooperativity in determining rates and pathways. In kinetic studies with consensus ankyrin repeat folding, we find multiple parallel pathways with a transition state that appears to have around 1.5-2 repeats folded but not yet paired (i.e., coupled). In contrast, we find that most naturally occurring repeat proteins fold through a single dominant pathway that corresponds to a region of lowest stability, and will illustrate this with folding and HX studies on a LRR protein. From the recurrent observation that the local free energy variation (1-2 kcal/mol) of naturally occurring repeat proteins is enough to collapse parallel folding into a single dominant pathway, it is tempting to generalize that folding by parallel pathways may be the exception rather than the rule, perhaps even more for globular proteins than for repeat proteins.

Contact Information

Dr. Frank Ferrone
(215) 895-2778
ferronfa@drexel.edu