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Laboratory Equipment and Instrumentation

Avestin Emulsiflex C-5 (Fermentation)

Fermentation Facilities for large-scale growth of bacterial and yeast cultures include eight temperature-controlled floor shakers, a walk-in 37°C chamber, and a 20-liter New Brunswick BioFlow IV fermenter (with a dedicated CEPA continuous flow centrifuge for harvesting cell pellets). An Avestin Emulsiflex C-5 is available for rapid and efficient breakage of cells for protein purification.

Location: Department of Biochemistry & Molecular Biology

Beckman Liquid Scintillation Counter

Beckman System Gold HPLC

Biacore 3000

Biosensor Technology Biacore 3000 surface plasmon resonance instrument, used for detecting interactions between biological macromolecules.

Location: Chaiken Research Group, Department of Biochemistry

BioRad QX-200 Digital Droplet PCR

BioRad QX-200 Digital Droplet PCR Location: Center for Advanced Microbial Processing (CAMP), 245 N. 15th Street

Capillary Electrophoresis

Capillary Electrophoresis Our Beckman Coulter P/ACE MDQ capillary electrophoresis system provides a versatile, efficient, and sensitive technique for the separation of biomolecules. The option exists to pipe the output from the CE system directly to our electrospray mass spectrometer.

Location: Department of Biochemistry & Molecular Biology

Eksigent NanoLC

Elevated Plus Maze Package with IR Beam for Rats (Med Associates)

Standard elevated plus maze with black walls and black runways to test anxiety.

Location: 2900 W. Queen Lane, Room 170, Barson Lab, Department of Neurobiology and Anatomy

Emax Plus Microplate Reader (Molecular Devices)

Eight filter modes enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA.

Location: 2900 W. Queen Lane, Room 170, Barson Lab, Department of Neurobiology and Anatomy

Extra Tall Modular Operant Test Chambers for Rats (Med Associates)

Chambers to test operant responding for liquid reinforcers.

Location: 2900 W. Queen Lane, Room 170, Barson Lab, Department of Neurobiology and Anatomy

Fluoromax-3 Fluorimeter

Fluoromax-3 Fluorimeter Shared facilities with the Department feature a Jasco J810 CD spectrometer and a Fluoromax-3 Fluorimeter (Spex).

Location: Department of Biochemistry & Molecular Biology

GE Akta FPLCs

Hi-Tech Scientific Stopped-Flow System (Spectroscopy)

Spectroscopy The Jorns laboratory operates a Hi-Tech Scientific Stopped-Flow System (SF-61DX2) that is equipped for anaerobic single- or double-mixing experiments with multiple detection options, including single wavelength and diode array absorbance modes.

Location: Department of Biochemistry & Molecular Biology

Inverted Epifluorescence Microscope

Inverted Epifluorescence Microscope In addition, the Department has an Olympus IX71 inverted epifluorescence microscope with MetaMorph/MetaFluor Imaging System, CoolSnap HQ Digital CCD camera, and DG4 Galvo-wavelength switcher as well as an Olympus AX70 Compound epifluorescence microscope with Spot RT Slider camera.

Location: Department of Biochemistry & Molecular Biology

Leica Laser Scanning Spectral Confocal Microscope

Fluorescence Microscopy The Reginato lab operates the confocal facility featuring the Leica Laser Scanning Spectral Confocal Microscope TCS SP2 AOBS advanced image acquisition and analysis system. This system includes a UV laser, as well as standard argon, and helium neon lasers that can simultaneously image four different fluorophores. In addition, the microscope includes objectives of 20x, 40x, 63x, and 100x magnification.

Location: The Reginato Lab

Mass Spectrometer

Mass Spectrometer Location: Center for Advanced Microbial Processing (CAMP), 245 N. 15th Street

MicroCal Vp-ITC Isothermal Titration Calorimeter

Microm HM525 NX Cryostat (Thermo Scientific)

A high performance routine cryostat with intuitive software and touchscreen for simple, efficient operation.

Location: 2900 W. Queen Lane, Room 170, Barson Lab, Department of Neurobiology and Anatomy

Nanostring

Nanostring Location: Center for Advanced Microbial Processing (CAMP), 245 N. 15th Street

Nextseq 500

Nextseq 500 Location: Center for Advanced Microbial Processing (CAMP), 245 N. 15th Street

Odyssey Infrared Imaging System

The department has acquired an Odyssey infrared imaging system from LI-COR Biosciences. This is a powerful technique that can enhance the sensitivity of western blots as well as a variety of other assays.

Location: Department of Biochemistry & Molecular Biology

Open Field Testing Environments for Rats

(w/ hole board floor inserts, two-chamber place preference inserts and light-dark box inserts) (Med Associates)

Animal movement is tracked through the use of three 16 beam IR arrays, which are located on both the X and Y axes for positional tracking and Z axis for rearing detection. Inserts allow for additional testing of novelty-seeking, reward/reinforcement and anxiety

Location: 2900 W. Queen Lane, Room 170, Barson Lab, Department of Neurobiology and Anatomy

Optical Reaction Module

Optical Reaction Module The Bio-rad CFX96™ optical reaction module converts a C1000™ thermal cycler into a powerful and precise real-time PCR detection system. This six-channel real-time PCR system comb has precise thermal control and delivers sensitive, reliable detection.

Location: Department of Biochemistry & Molecular Biology

PacBio DNA Sequencer

PacBio DNA Sequencer Location: Center for Advanced Microbial Processing (CAMP), 245 N. 15th Street

Perkin Elmer Filter Mate Universal Harvester

Perkin Elmer Filter Mate Universal Harvester Harvests or filters cell preparations (such as transfected or stable cell lines or synaptosomes) for neurotransmitter uptake and binding assays.

Users: Pharmacology, Neuroscience and Biochemistry Students

Location: New College Building, Room 8405, Andreia Mortensen Lab, Department of Pharmacology and Physiology

ProteOn™ XPR36 Protein Interaction Array System

Protein Interaction Array System The ProteOn™ XPR36 protein interaction array system is an SPR optical biosensor that provides real-time data on the affinity, specificity, and interaction kinetics of protein interactions. Using XPR technology, a unique approach to multiplexing, this system generates a 6 x 6 interaction array for the simultaneous analysis of up to six ligands with up to six analytes. The ProteOn XPR36 system increases the throughput, flexibility, and versatility of experiment design, enabling the completion of more experiments in less time.

Location: Department of Biochemistry & Molecular Biology

Seahorse Analyzer XFp Analyzer

Seahorse Analyzer XFp Analyzer This instrument measures cellular metabolism in live cells in real time, via the measurement of extracellular flux (oxygen consumption rate and extracellular acidification rate) to provide insight into mitochondrial function and glycolytic activity. XF technology can be used to determine which pathway is predominant (metabolic phenotype), as well as which substrates are being used for energy production. Glucose oxidation, fatty acid oxidation, and aerobic glycolysis rates can be readily measured under multiple conditions.

Location: Department of Biochemistry & Molecular Biology

Spectrophotometer (Nanodrop)

Full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, and protein.

Location: 2900 W. Queen Lane, Room 170, Barson Lab, Department of Neurobiology and Anatomy

StepOne Plus RT-PCR System (Applied Biosystems)

A 96-well Real-Time PCR instrument utilizing robust LED based 4-color optical recording, and designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Location: 2900 W. Queen Lane, Room 170, Barson Lab, Department of Neurobiology and Anatomy

TissueLyser II (Qiagen)

Simultaneously disrupts multiple biological samples through high-speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. Alternatively, a grinding jar set can be used to process large samples.

Location: 2900 W. Queen Lane, Room 170, Barson Lab, Department of Neurobiology and Anatomy

Typhoon FLA 700 Biomolecular Imaging System

Varian Preparative-Scale HPLC With Automated Sample Changer

Virtis Benchtop 4.0 ZL Lyophilizer

 
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