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Harpreet Singh

Harpreet Singh, PhD, MSc

Associate Professor


Department: Pharmacology & Physiology, Medicine: Division of Cardiology

Education

  • Postdoctoral Fellowship – University of California Los Angeles (2011)
  • PhD in Biomedical Sciences - Centre for Integrative Physiology, University of Edinburgh, U.K. (2007)
  • MSc in Biotechnology - JB Campus, Bangalore University, India (2003)
  • BSc in Chemistry, Microbiology and Zoology - St Joseph’s College, Bangalore, India (2001)

Harpreet Singh, PhD, is an associate professor in the Department of Pharmacology and Physiology at Drexel University College of Medicine.

Research Overview

Dr. Singh earned his PhD from the University of Edinburgh, U.K., in 2007, followed by postdoctoral training at the University of California Los Angeles. He worked as a research faculty member at UCLA for two years before joining the faculty of Drexel University College of Medicine in 2013.

Research Interests

Intracellular ion channels and their role in cardiac function and aging

Research

Ion channels present on the plasma membrane facilitate passive diffusion of ions down their electrochemical gradient. They generate electrical impulses underlying neuronal transmission, hormone secretion and control of heartbeat. In addition to the plasma membrane, ion channels are also present on the membranes of intracellular organelles, where they regulate ionic homeostasis in cellular organelles, play a role in acidification of intracellular compartments, maintain intracellular calcium concentrations and membrane potential, participate in muscle contraction and also facilitate ATP generation.

Research in the Singh Laboratory is focused on understanding intracellular ion channels in the heart and their role on heart function during aging, in particular cardiac mitochondrial ion channels and ChLoride Intracellular Channels (CLICs). The laboratory studies molecular identity, structure-function, and regulation of cardiac intracellular ion channels by physiological pathways, pharmacological agents, their role in coordination of ionic homeostasis and molecular signaling. DNA probes encoding intracellular channels, site-directed mutagenesis, purified proteins, high-resolution imaging, and functional expression are used to assess the molecular mechanisms involved in intracellular ion channel functioning.

Publications

Recent Selected Publications
View complete list of publications

“Identification and Characterization of a Bacterial Homolog of Chloride Intracellular Channel (CLIC) Protein Family”
Gururaja Rao S, Ponnalagu D, Sukur S, Singh H, Sanghvi S, Mei Y, Jin D, and H Singh
Scientific Reports, in press (2017)

"Anion Channels of Mitochondria"
Ponnalagu D, and H Singh
Pharmacology of Mitochondria. Handbook of Pharmacology
, Springer Publishers, in press (2016)

"Comprehensive echocardiographic assessment of the right ventricle in murine models"
Kohut A, Patel N, and H Singh
Jour. of Cardiovascular Ultrasound (JCU), in press (2016)

"Optimization of Non-Thermal Plasma Treatment in an in vivo Model Organism" 
Lee A, Lin A, Shah K, Singh H, Miller V. and S Gururaja Rao.
PLoS One, e0160676 (2016)

"Skeletal muscle ERα action is critical for the maintenance of mitochondrial function and metabolic homeostasis in females"       
Ribas V, Drew BG, Zhou Z, Phun J, Kalajian NY, Soleymani T, Daraei P, Widjaja K, Wanagat J, de Aguiar Vallim TQ, Fluitt AH, Bensinger S, Le T, Radu C, Whitelegge JP, Beaven SW, Tontonoz P, Lusis AJ, Parks BW, Vergnes L, Reue K, Singh H, Bopassa JC, Toro L, Stefani E, Watt MJ, Schenk S, Akerstrom T, Kelly M, Pedersen BK, Hewitt SC, Korach KS, and A.L Hevener.
Science Translational Medicine, 8(334):334ra54 (2016)

"Data supporting the specificity of CLIC1, CLIC4, CLIC5 and DmCLIC antibodies and their localization in endoplasmic reticulum of cardiomyocytes"         
Ponnalagu D, Gururaja Rao S, Farber J, Xin W, Hussain AT, Shah K, Tanda S, Edwards J C, Berryman M, and H. Singh
Data in Brief, 7:1038–1044 (2016)

"Molecular Identity of Cardiac Mitochondrial Chloride Intracellular Channels"        
Ponnalagu D, Gururaja Rao S, Farber J, Xin W, Hussain A.T, Shah K, Tanda S, Edwards J C, Berryman M, Bopassa J.C, and H. Singh
Mitochondrion, 27:6-14. (2016)                    

"MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain"       
#Singh H, Li M, Chen S, Sukur S, Lu R, Caputo A, Meredith A.L, Stefani E, and LToro
Neuroscience, 317:76-107 (2016)
#corresponding author

"G protein-Coupled Estrogen Receptor 1 Mediates Acute Estrogen-Induced Cardioprotection via MEK/ERK/GSK-3b pathway after Ischemia/Reperfusion"
Kabir M.E; Singh H.; Lu R.; Olde B.; Leeb-Lundberg L. M. F.; and J. C. Bopassa
PloS One, 10(9):e0135988 (2015)

"Regulation of Renal Hemodynamics and Function by RGS2"
Osei-Owusu P., Owens E. A., Jie L., Reis J. S., Forrester S. J., Kawai T., Eguchi S., Singh H., and K. J. Blumer
PloS One, 10(7):e0132594 (2015)

"MaxiK channel and cell signalling."
Toro L, Li M, Zhang Z, Singh H, Wu Y, and Stefani E
Pflugers Arch. 466(5):875-86 (2014)

"Kcnma1 Gene Encodes mitoBKCa and a Spliced Exon Targets its α-subunit to Cardiac Mitochondria"
Singh H, Lu R, Bopassa JC, Meredith A, Stefani E, and L Toro
Proc. Natl. Acad. Sci. USA. 110:26;10836-10841 (2013)

"Response to Pomozi et al's research commentary."
Martin LJ , Lau E, Singh H, Vergnes L, Tarling EJ, Mehrabian M, Mungrue I, Xiao S, Shih D, Castellani L, Ping P, Reue K, Stefani E., Drake TA, Bostrom K, and Lusis AJ
Circulation Research. 24;112(11):e152-3 (2013)

"Intracellular BKCa (iBKCa) channels"
Singh H, Stefani E, and L Toro
J Physiology, 590:5937-5947 (2012)

"Building a Fast Scanning Stimulated Emission Depletion Microscope: A Step by Step Guide"
Rodríguez PFG, Wu Y, Singh H, Zhao H, Toro L, and E Stefani
Current Microscopy Contributions to Advances in Science and Technology. 2:791-800 (2012)

"Abcc6 localizes to the Mitochondria-Associated Membrane"
Martin LJ, Lau E, Singh H, Vergnes L, Tarling EJ, Mehrabian M, Mungrue I, Xiao S, Shih D, Castellani L, Ping P, Reue K, Stefani E, Drake TA, Bostrom K, and AJ Lusis
Circulation Research. 111:516-520 (2012)

"Visualization and quantification of cardiac mitochondrial proteins clusters with STED microscopy"
Singh H, Lu R, Rodriguez PFG, Wu Y, Bopassa JC, Stefani E, and L Toro
Mitochondrion. 12:230-236 (2012)

"Two decades with dimorphic ChLoride Intracellular Channels (CLICs)"
Singh H
FEBS letters. 584, 10:2112-2121 (2010)

"Proteomics to identify proteins interacting with P2X2 ligand-gated cation channels"
Singh H, Warburton S, Vondriska TM, and BS. Khakh
J. Vis. Exp. 27. doi: 10.3791/1178 (2009)

"Functional reconstitution of mammalian "chloride intracellular channels" CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin"
*Singh H, Cousin MA, and RH Ashley (*corresponding author)
FEBS J. 274: 6306-6316 (2007)

"CLIC4 (p64H1) and its putative transmembrane domain form poorly selective, redox-regulated ion channels"
Singh H, and RH Ashley
Mol. Membr. Biol. 24:41-52 (2007)

"Redox regulation of CLIC1 by cysteine residues associated with the putative channel pore."
Singh H, and RH Ashley
Biophys J. 90:1628-38 (2006)


Contact Information


Research Office

Department of Pharmacology & Physiology
245 N. 15th Street
Mail Stop 488
Philadelphia, PA 19102
Phone: 215.762.4751
Fax: 215.762.2299