For a better experience, click the Compatibility Mode icon above to turn off Compatibility Mode, which is only for viewing older websites.

Kazuhito Toyooka

Kazuhito Toyooka, PhD

Assistant Professor


Department: Neurobiology & Anatomy

Education

  • PhD in Immunology - Osaka University, Japan
  • Shizuoka University, Japan

Other Languages Spoken

Japanese

Dr. Toyooka is an assistant professor in the Department of Neurobiology & Anatomy at Drexel University College of Medicine. He did postdoctoral fellowships at the University of California San Diego and the University of California San Francisco. He also served on the faculty at Osaka City University School of Medicine in Japan. He was appointed to the faculty in the Department of Neurobiology & Anatomy at the College of Medicine in 2013.

He did postdoctoral fellowships at the University of California San Diego and the University of California San Francisco. He also served on the faculty at Osaka City University School of Medicine in Japan. He was appointed to the faculty in the Department of Neurobiology & Anatomy at the College of Medicine in 2013. - See more at: http://www.drexelmed.edu/Home/AboutOurFaculty/KazuhitoToyooka.aspx#sthash.a5rQ7400.dpuf

 

Research Overview

Current lab personnel:

  • Brett T. Cornell, PhD candidate

Past lab personnel:

  • Tomoka Wachi, PhD, postdoctoral fellow
  • Thomas Sibert, MD student
  • Courtney Marshall, PhD candidate

Research Interests

Neuronal development and neuronal migration

Research

Dr. Toyooka's lab studies the mechanisms of neuronal migration in the developing cerebral cortex and hippocampus. To uncover these mechanisms, they have focused on the 14-3-3 proteins.

Neurogenesis and neuronal migration require the precise choreography of more than 100 billion neuronal cells that form appropriate connections during brain development. However, the disruption of neurogenesis and neuronal migration results in a wide range of diseases, including brain morphological disorders such as lissencephaly and Miller-Dieker syndrome (MDS) and mental illnesses such as autism and schizophrenia. The lab's goals are to understand the mechanisms of neuronal migration that are required to form appropriate neuronal connections and to clarify the roles of the multifunctional 14-3-3 protein family in brain development and behaviors in conjunction with human diseases. To achieve these goals, they have been using multiple approaches including mouse genetic techniques and time-lapse live imaging using brain slices.

Ongoing Projects

  • The analysis of the functions of the 14-3-3 proteins in neuronal migration in the cerebral cortex and the hippocampus
  • The understanding of the etiology of lissencephaly/Miller-Dieker syndrome
  • The analysis of the functions of the 14-3-3 proteins in behaviors in conjunction with human mental illnesses such as autism and schizophrenia

Mechanisms of neuronal migration in the developing cerebral cortext and hippocampus

Publications

Peer-Reviewed Manuscripts

"Overexpression of the 14-3-3Gamma Protein in Embryonic Mice Results in Neuronal Migration Delay in the Developing Cerebral Cortex"
Cornell B, Wachi T, Zhukarev V and Toyo-oka K
Neurosci Lett, in press, doi:10.1016/j.neulet.2016.06.009.

"Deficiency of 14-3-3ε and 14-3-3ζ by the Wnt1 promoter-driven Cre recombinase results in pigmentation defects"
Cornel B and Toyo-oka K
BMC Res Notes, 9(1);180-185, 2016

"Ablation of the 14-3-3gamma Protein Results in Neuronal Migration Delay and Morphological Defects in the Developing Cerebral Cortex"
Wachi T, Cornell B, Marshall C, Zhukarev V, Baas PW, and Toyo-Oka K
Dev Neurobiol., 76 (6): 600-614, 2016

"14-3-3epsilon and zeta Regulate Neurogenesis and Differentiation of Neuronal  Progenitor Cells in the Developing Brain""
Toyo-oka K, Wachi T, Hunt RF, Baraban SC, Taya S, Ramshaw H, Kaibuchi K,  Schwarz QP, Lopez AF and Wynshaw-Boris A
J Neurosci., 34(36):12168-12181, 2014

"14-3-3{varepsilon} Plays a Role in Cardiac Ventricular Compaction by Regulating the Cardiomyocyte Cell Cycle"
Kosaka Y, Cieslik KA, Li L, Lezin G, Maguire CT, Saijoh Y, Toyo-Oka K, Gambello MJ, Vatta M, Wynshaw-Boris A, Baldini A, Yost HJ and Brunelli L
Mol. Cell. Biol., 32:5089-5102, 2012

"Neurodevelopmental defects and neuropsychiatric behaviour arise from 14-3-3zeta deficiency"
Cheah PS, Ramshaw HS., Thomas PQ, Toyo-oka K, Martin S, Coyle P, Guthridge MA, Stomski F, van den Buuse M, Wynshaw-Boris A, Lopez AF and Schwarz QP
Mol. Psychiatr., 17:451-466, 2011

"Identification of YWHAE, a gene encoding 14-3-3epsilon, as a possible susceptibility gene for schizophrenia"
Ikeda M, Hikita T, Taya S, Uraguchi-Asaki J, Toyo-oka K, Wynshaw-Boris A, Ujike H, Inada T, Takao K, Miyakawa T, Osaki N, Kaibuchi K and Iwata N
Hum. Mol. Genet., 17: 3212-3222, 2008

"A Neuroepithelial Stem Cell Proliferation requires LIS1 for Precise Spindle Orientation and Symmetric Division"
Yingling J, Youn YH, Darling D, Toyo-oka K, Pramparo T, Hirotsune S and Wynshaw-Boris
Cell, 132: 474-486, 2008

"Protein Phosphatase4 catalytic subunit (PP4c) regulates CDK1 activity and organization of microtubules through dephosphorylation of NDEL1"
Toyo-oka K, Yano Y, Shiota M, Iwao H, Hiraiwa N, Muramatsu M, Yoshiki A and Hirotsune S
J Cell Biol., 180: 1133-1147, 2008

"NDEL1 Phosphorylation by Aurora-A Kinase Is Essential for Centrosomal Maturation, Separation, and TACC3 recruitment"
Mori D, Yano Y, Toyo-oka K, Yoshida N, Yamada M, Muramatsu M, Zhang D, Saya H, Toyoshima YY, Kinoshita K, Wynshaw-Boris A and Hirotsune S
Mol. Cell. Biol., 27: 352-367, 2007

"Mnt-Deficient Mammary Glands Exhibit Impaired Involution and Tumors with Characteristics of Myc Overexpression"
Toyo-oka K, Bowen TJ, Hirotsune S, Li Z, Jain S, Ota S, Lozach LE, Bassett IG, Lozach J, Rosenfeld MG, Glass CK, Eisenman R, Ren B, Hurlin PJ and Wynshaw-Boris A
Cancer Res., 66: 5565-5573, 2006

"Recruitment of katanin P60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration"
Toyo-oka K, Sasaki S, Yano Y, Mori D, Kobayashi T, Toyoshima YY, Tokuoka SM, Ishii S, Shimizu T, Muramatsu M, Hiraiwa N, Yoshiki A, Wynshaw-Boris A and Hirotsune S
Hum. Mol. Genet., 14: 3113-3128, 2005

"Complete Loss of Ndel1 Results in Neuronal Migration Defects and Early Embryonic Lethality"
Sasaki S, Mori D, Toyo-oka K, Chen A, Garrett-Beal L, Muramatsu M, Miyagawa S, Hiraiwa N, Yoshiki A, Wynshaw-Boris A, and Hirotsune A
Mol. Cell. Biol., 25:7812-7827, 2005

"Loss of the Max-interacting protein Mnt in mice results in decreased viability, defective embryonic growth and craniofacial defects: relevance to Miller-Dieker syndrome"
Toyo-oka K, Hirotsune S, Gambello MJ, Zhou Z-Q, Olson L, Rosenfeld MG, Eisenman R, Hurlin PJ and Wynshaw-Boris A
Hum. Mol. Genet., 13:1057-1067, 2004

"Evidence of Mnt-Myc Antagonism Revealed by Mnt Gene Deletion"
Hurlin PJ, Zhou Z-Q, Toyo-oka K, Ota S, Walker WL, Hirotsune S and Wynshaw-Boris A
Cell Cycle, 3:97-99, 2004

"Deletion of Mnt leads to disrupted cell cycle control and tumorigenesis"
Hurlin PJ, Zhou Z-Q, Toyo-oka K, Ota S, Walker WL, Hirotsune S and Wynshaw-Boris A
EMBO J., 22:4584-4596, 2003

"14-3-3epsilon is important for neuronal migration via binding of NUDEL : a molecular explanation for Miller-Dieker syndrome"
Toyo-oka K, Shionoya A, Gambello MJ, Cardoso C, Leventer R, Ward HL, Ayala R, Tsai L-H, Dobyns W, Ledbetter D, Hirotsune S and Wynshaw-Boris A
Nat. Genet., 34:274-285, 2003

"Refinement of a 400-kb Critical Region Allows Genotypic Differentiation between Isolated Lissencephaly, Miller-Dieker Syndrome, and Other Phenotypes Secondary to Deletions of 17p13.3"
Cardoso C, Leventer RJ, Ward HL, Toyo-oka K, Chung J, Gross A, Martin CL, Allanson J, Pilz DT, Olney AH, Mutchinick OM, Hirotsune S, Wynshaw-Boris A, Dobyns WB and Ledbetter DH
Am. J. Hum. Genet., 72:918-930, 2003

Reviews

"Miller-Dieker Syndrome: Analysis of a Human Contiguous Gene Syndrome in the Mouse"
Yingling J, Toyo-oka K, and Wynshaw-Boris A
Am. J. Hum. Genet., 73(3):475-488, 2003


Contact Information


Research Office

Department of Neurobiology & Anatomy
2900 W. Queen Lane, Room 186
Philadelphia, PA 19129
Phone: 215.991.8288