The Department of Neurobiology and Anatomy is located in
20,000 square feet of newly renovated laboratory space at the Queen Lane Campus of Drexel University College of Medicine. The Queen Lane Campus
also houses the Department of Microbiology and Immunology and the teaching
facilities for medical students.
The Department of Neurobiology & Anatomy imaging facilities include:
- Automated wide-field fluorescence microscope for multi-color image acquisition
- Stereology system equipped with Neurolucida neuron tracing software
- Laser microdissection system for isolating specific cells from tissue samples
- Automated four-quadrant spectrophotometer plate reader for protein interaction assays
- High-end spectral confocal microscope with visible and ultra-violet illumination capabilities and corresponding software allowing for virtually artifact-free multi-color fluorescence image acquisition and analysis
For additional information, training and reservations of the imaging facilities, contact Dr. Vladimir Zhukarev at firstname.lastname@example.org.
The Queen Lane Library
on the main floor of the Drexel University College of Medicine
Campus (2900 W. Queen Lane), houses books, journals and other materials to
support the educational needs of students in the first two years of
medical school, and the research needs of the basic sciences departments.
The collection focuses on the basic sciences, including research
literature in biochemistry, microbiology/immunology, neuroscience,
physiology, molecular biology and cell biology; there is also a core
collection of books on clinical medicine. Approximately 17,000 books and
journals and over 200 videotapes and slide sets are available.
Drexel University College of Medicine has
developed a reputation for excellence in medical education and research.
Together, the Departments of Neurobiology and Anatomy, Pharmacology and
Physiology, Neurology, Neurosurgery and Psychiatry form an
interdisciplinary core of scientists involved in neuroscience research.
Institutional agreements make some software available, via Drexel FTP server,
to members of the Drexel community.
Visit the Drexel software portal. (If the link does not work the first time,
click on refresh or reload.)
In order to download the software, you
must Log in with your Drexel domain user ID and password. Enter your user ID
Suggestions, additions, deletions please contact Francis Sessler.
This page is to help all of us conform to the continually updated and changing
university regulations. In addition contact numbers and procedures on how
to get things done are included.
Jonathan M. Chase,
Exectuive Director, Department of Environmental Health and Safety,
of Research Policy and Procedures, Manuals and Guidelines
- IACUC Policies
- IRB Guidelines
- General Laboratory Safety
- Right to Know Guidelines
- Research Conduct Policy
Drexel's Chemical Hygiene Plan (to comply with OSHA's Health and Safety Standards)
Sigma-Aldrich company family material safety data sheets
MSDS Search Engine
Hazardous Material Identification Guide
The NFPA Fire Diamond
Getting Things Done
Sharps and biohazard removal is performed by the College of Medicine.
Sharps containers are available FREE from Department of Environmental Health and Safety.
Hoods need to be calibrated regularly (yearly).
This service is scheduled by the Department of Environmental Health and Safety.
Hoods down? No DI? Water brown?
Contact Facilities Management:
Director: Dr. Vladimir Zhukarev
The confocal facility consists of the Leica TCS SP2 VIS/UV confocal microscope with semi-automatic upright optical stand and dual monitor Windows XP computer workstation. The facility is housed in Room 251 on the second floor of Queen Lane, in the Department of Neurobiology and Anatomy.
The director, Dr. Zhukarev, trains all new users, oversees day-to-day operation, maintains the facility and updates the reservation calendar and schedule of microscope use. Dr. Zhukarev supervises the overall operation by establishing rules and procedures for the use of the microscope. All problems or issues that arise with the microscope are to be directed to Dr. Zhukarev.
In the long run Dr. Zhukarev will also participate in specific research projects that require complex image acquisition and analysis as specified below.
- Use of the microscope is restricted to investigators within the Department of Neurobiology and Anatomy. People from outside of the department who wish to use the system must receive authorization from the department chair, Dr. Itzhak Fischer.
- All users must obtain training prior to unsupervised use of the microscope, even if other workers from the same laboratory have already been trained or the user has had prior experience with a similar microscope.
- The microscope is available to eligible users after training within the proper procedures and guidelines.
- Access to the facility is allowed between 9 a.m. and 6 p.m. Monday through Friday. Special authorization is required to access the facility during weekends or outside of the normal hours of operation.
- Individual researchers are responsible for conducting their own imaging sessions on the microscope. Problems while using the microscope should be recorded in the log journal. It is important to keep in mind that failure to do so creates the potential of serious hardware breakdown with very expensive repair as a result.
All training of new users will be conducted by Dr. Zhukarev only. Principal investigators should contact the director to request training of their students.
Help and Collaboration of Research Projects
Dr. Zhukarev will be available to help with research projects in both the acquisition of high resolution images and in image processing steps for all microscope platforms, including the two-photon and the super-resolution microscopes (at Biology).
Arrangement for research help and collaboration are encouraged, but must be approved by the department chair, Dr. Itzhak Fischer.
Reserving the Confocal Microscope
Users should book time on the microscope up to one week in advance; cancellation of planned usage should be made in timely fashion. The Google calendar application that is provided free for Gmail customers is the most user-friendly way to make reservations. It is expected that all confocal microscope facility users will provide their email address to have access to the calendar in sharing mode. While defining a time slot, users should also enter: 1) user name, 2) very brief description of the imaging experiment and 3) the name of PI.
Example: Joe Somebody; spinal cord tissue slices, fixed specimen, DAPI/Cy3/Cy5 fluorescence; Dr. Tom.
Unless there are urgent needs (e.g., before major meetings such as SFN), the time slot duration can be as long as the full working day hours of the facility (i.e., 9 a.m. - 6 p.m.). Exception is for live specimen experiments, when the microscope can be booked for several consecutive days. Due to safety regulations overnight, unsupervised imaging is not permitted.
Under no circumstances may a user sign up for time using another person's name.
Image Facility Rules
- Users will obtain a key for the facility from the office of the Department of Neurobiology and Anatomy.
- Users should record their actual log-in and log-out time in the microscope log journal located on the workstation desk.
- Before turning the system on, users must turn on the air conditioning unit using the remote control. The very last user of the day should turn it off before leaving the room.
- Entering the room wearing protective gloves is not permitted. Food and drinks are not allowed.
- Before starting their session, users must clean old oil off their slides using 70% alcohol. Mixture of oils either on the slide or on the oil lenses (where it will end up staying) corrupts the quality of image acquisition.
- Spills must be taken care of immediately and reported in the log journal for further technical care.
- At the end of each session, oil objective lenses must be gently blotted by a stretched Kimwipe.
- If the microscope is not scheduled to be used again for more than one hour after a current session is over (check the Google calendar schedule), all components of the microscope should be shut down according to the posted shutdown procedure. If the interval is shorter, the user needs to close the Leica software program and to log out leaving the confocal microscope hardware running.
- The last user of the day is responsible for ensuring that the microscope is shut down properly.
The workstation runs the Windows XP operating system that is no longer supported by Microsoft. Because of that, the workstation is going to be kept separated from the institutional network.
Users are not allowed to download or install software on the computer.
Users should back up their files on CD/DVD or other storage medium, and delete their files from the hard disk. Any user data remaining on the hard drive will be removed during routine maintenance by the end of each month without notifying users.
Cancellation of User Privileges
Because this is shared use facility, repeated violation of the stated rules and procedures will not be tolerated. Perpetrators will be subject to loss of privileges at the discretion of the facility director.